25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibody and antigen (hapten, BSA/OVA conjugated)

Diagnostic anti-25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibodies (anti-25 hydroxyvitamin D, anti-25-OH-(VD3+VD2)) and diagnostic hapten-carrier conjugates BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2)), OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2)), KLH-25 hydroxyvitamin D (25-OH-(VD3+VD2)) antigens for Metabolic diseases 25-OH-(VD3+VD2) detection in ELISA test, competitive ELISA, Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT.

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-SMT-25-OH-(VD3+VD2)-Ag01	BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2))	756
GMP-SMT-25-OH-(VD3+VD2)-Ag02	OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2))	756
GMP-SMT-25-OH-(VD3+VD2)-Ab01	Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) rabbit monoclonal antibody (rab mAb)	1953
GMP-SMT-25-OH-(VD3+VD2)-Ab02	Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) rabbit monoclonal antibody (rab mAb)	1953

Size： 1mg | 10mg | 100mg

Product Description

Cat No. of Products	GMP-SMT-25-OH-(VD3+VD2)-Ag01
Product Name	BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2))
Target/Biomarker	25 hydroxyvitamin D(25-OH-(VD3+VD2))
Expression platform	Synthetic
Bioactivity validation	25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 25-OH-(VD3+VD2) level test of Metabolic diseases (bone fracture, coronary heart disease, cancer, and all-cause mortality) and related syndrome evaluation.
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) / OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) is Synthetic.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and immunonephelometry.
Formulation	Supplied as a 0.2 μM filtered solution of PBS,PH7.4.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-SMT-25-OH-(VD3+VD2)-Ag02
Product Name	OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2))
Target/Biomarker	25 hydroxyvitamin D(25-OH-(VD3+VD2))
Expression platform	Synthetic
Bioactivity validation	25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 25-OH-(VD3+VD2) level test of Metabolic diseases (bone fracture, coronary heart disease, cancer, and all-cause mortality) and related syndrome evaluation.
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) / OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) is Synthetic.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and immunonephelometry.
Formulation	Supplied as a 0.2 μM filtered solution of PBS,PH7.4.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-SMT-25-OH-(VD3+VD2)-Ab01, GMP-SMT-25-OH-(VD3+VD2)-Ab02
Product Name	Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) rabbit monoclonal antibody (rab mAb)
Target/Biomarker	25 hydroxyvitamin D(25-OH-(VD3+VD2))
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other 25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibodies in 25-OH-(VD3+VD2) level test of Metabolic diseases (bone fracture, coronary heart disease, cancer, and all-cause mortality) and related syndrome evaluation.
Tag	mFc
Products description	Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) monoclonal antibody (mAb) is a monoclonal antibody produced by hybridoma technology.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA),Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and immunonephelometry.
Formulation	Supplied as a 0.2 μM filtered solution of PBS,PH7.4.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Figure 1: Total 25-OH-VD identification ability
Figure 2: Sample coincidence rate
Figure 3: Standard curve
Figure 4: Clinical comparative analysis

Figure 1. Total 25-OH-VD recognition ability; As can be seen from the figure above, 25-OH-VD2 and 25-OH-VD3 exhibit good cross-reactivity.

Genemedi's 25-OH-VD sandwich antibodies (GMP-SMT-25-OH-(VD3+VD2)-Ab01 and GMP-SMT-25-OH-(VD3+VD2)-Ab02) were validated on the direct chemiluminescence platform. The total amount of 25-OH-VD in the sample may be successfully detected thanks to the good cross-reactivity of 25-OH-VD2 and 25-OH-VD3.

Figure 2. Self-assessment clinical comparative analysis and Clinical comparative analysis of customer testing. The antibodies showed excellent agreement compared to mass spectrometry detection results, with an R2 as high as 0.9718 and a customer-validated R2 of 0.963. The sandwich approach greatly improves the accuracy of immunoassays.

The VD sandwich antibody(GMP-SMT-25-OH-(VD3+VD2)-Ab01 and GMP-SMT-25-OH-(VD3+VD2)-Ab02) made by Genemedi was validated using a direct chemiluminescence platform with the industry's gold standard LC-MS/MS as a control to increase the accuracy of the 25-OH-VD immunoassay. When compared to the mass spectrometry detection results, these antibodies exhibited excellent consistency, with an R2 of up to 0.9718 and a customer-verified R2 of 0.963. The sandwich approach greatly improved immunodetection accuracy.

Figure 3. Standard curve. As can be seen from the figure above, Genemedi's 25-OH-VD sandwich antibody has a detection concentration range of 0.1 to 80ng/mL.

The detection concentration range for Genemedi's 25-OH-VD sandwich antibodies (GMP-SMT-25-OH-(VD3+VD2)-Ab01 and GMP-SMT-25-OH-(VD3+VD2)-Ab02) is 0.1 to 80ng/mL.

Figure 4. Clinical comparative analysis(GMP-SMT-25-OH-(VD3+VD2)-Ab01 and GMP-SMT-25-OH-(VD3+VD2)-Ab02).

As can be seen from the figure above, Detection employing 25-OH-VD sandwich antibodies validated by an enzymatic chemiluminescence platform using an R2 of 0.9741, the data demonstrated strong consistency using mass spectrometry (LC-MS/MS).

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Target/Biomarker information

Vitamin D is a nutrient that is essential for healthy bones and teeth. There are two forms of vitamin D that are important for nutrition: vitamin D2 and vitamin D3. Vitamin D2 mainly comes from fortified foods like breakfast cereals, milk, and other dairy items. Vitamin D3 is made by your own body when you are exposed to sunlight. It is also found in some foods, including eggs and fatty fish, such as salmon, tuna, and mackerel. In your bloodstream, vitamin D2 and vitamin D3 are changed into a form of vitamin D called 25 hydroxyvitamin D, also known as 25(OH)D. A vitamin D blood test measures the level of 25(OH)D in your blood. Abnormal levels of vitamin D can indicate bone disorders, nutrition problems, organ damage, or other medical conditions. A vitamin D test is used to screen for or monitor bone disorders. It is also sometimes used to check vitamin D levels in people with chronic illnesses such as asthma, psoriasis, and certain autoimmune diseases.

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